Media Storage Type : DVD-ROM
NPTEL Course Name : Plant Biotechnology
NPTEL Subject Matter Expert : Dr. Rakhi Chaturvedi
NPTEL Co-ordinating Institute : IIT Guwahati
NPTEL Lecture Count : 42
Lecture Titles:
Lecture 1 - Introduction to plant tissue culture
Lecture 2 - Lab facilities and operations
Lecture 3 - Tissue Culture Media
Lecture 4 - Plant Tissue culture techniques
Lecture 5 - Micropropagation by adventitious shoot proliferation
Lecture 6 - Micropropagation by Axillary shoot proliferation
Lecture 7 - Meristem culture for virus elimination
Lecture 8 - Somatic Embryogenesis and Artificial seed production
Lecture 9 - Doubled Haploid production
Lecture 10 - Triploid production by Endosperm culture
Lecture 11 - Cell suspension cultures
Lecture 12 - Protoplast Isolation and Regeneration
Lecture 13 - Somatic Hybridization and Cybridization
Lecture 14 - Somaclonal, Protoclonal and Gametoclonal variation
Lecture 15 - Cryopreservation
Lecture 16 - Cytological and various staining procedures for Ploidy analysis
Lecture 17 - Microscopy
Lecture 18 - Electron Microscopy
Lecture 19 - Flow Cytometry and cell sorting
Lecture 20 - Plant Histological Techniques
Lecture 21 - Genetic material of plant cells
Lecture 22 - Restriction Enzymes
Lecture 23 - Plant transformation and transformation vectors
Lecture 24 - Mode of Gene delivery in plant
Lecture 25 - Agrobacterium mediated Gene transfer
Lecture 26 - Ti and Ri Plasmids
Lecture 27 - Selection and screening of transformations
Lecture 28 - PCR and Hybridization technique
Lecture 29 - Gene silencing
Lecture 30 - Applications of Genetic Engineering
Lecture 31 - Advantages of Plant cell, Tissue and organ culture as source of secondary metabolites
Lecture 32 - Hairy Root cultures
Lecture 33 - Screening of High yielding cell lines and extraction of High value Industrial Products
Lecture 34 - Fractionation and Bioassays of plant extract
Lecture 35 - Growth and Production Kinetics of Cell cultures in Shake Flasks
Lecture 36 - Bioreactors for Plant Engineering
Lecture 37 - Manipulation in Production Profile by Abiotic and Biotic Elicitation
Lecture 38 - Biotransformation
Lecture 39 - Aims Scope and Bottlenecks of Molecular Farming
Lecture 40 - Production of Industrial Enzymes and Biodegradable Plastics
Lecture 41 - Production of Antibodies
Lecture 42 - Metabolic Engineering for Production of Fatty Acids, Industrial Oils, Terpenoids and Flavonoids