Media Storage Type : 32 GB USB Stick
NPTEL Subject Matter Expert : Prof. Sanjeeva Srivastava
NPTEL Co-ordinating Institute : IIT Bombay
NPTEL Lecture Count : 40
NPTEL Course Size : 16 GB
NPTEL PDF Text Transcription : Available and Included
NPTEL Subtitle Transcription : Available and Included (SRT)
Lecture Titles:
Lecture 1 - Introduction to amino acids
Lecture 2 - Introduction to proteins
Lecture 3 - Protein folding and misfolding
Lecture 4 - Introduction to proteomics
Lecture 5 - Lab session – Protein-protein interaction using label-free biosensors
Lecture 6 - Sample preparation and pre-analytical factors
Lecture 7 - Sample preparation: Pre-analytical factors (Continued...)
Lecture 8 - Sample preparation: Protein extraction and quantification
Lecture 9 - One-dimensional electrophoresis
Lecture 10 - Introduction to 2-DE
Lecture 11 - 2-DE: Second dimension, staining and destaining
Lecture 12 - 2-DE: Gel analysis
Lecture 13 - 2-DE Applications
Lecture 14 - 2-DE Applications (Continued...) and Challenges
Lecture 15 - Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR and data analysis
Lecture 16 - 2D-DIGE: Basics
Lecture 17 - 2D-DIGE: Data analysis
Lecture 18 - 2D-DIGE: Applications
Lecture 19 - Systems biology and proteomics - I
Lecture 20 - Systems biology and proteomics - II
Lecture 21 - Fundamentals of mass spectrometry
Lecture 22 - Chromatography technologies
Lecture 23 - Liquid chromatography
Lecture 24 - Mass spectrometry: Ionization sources
Lecture 25 - Mass spectrometry: Mass analyzers
Lecture 26 - MALDI sample preparation and analysis
Lecture 27 - Hybrid mass spectrometry configurations
Lecture 28 - Lab session - Demonstration of Q-TOF MS technology
Lecture 29 - In-gel and in-solution digestion
Lecture 30 - Lab session - Sample preparation: tissue sample preservation technology
Lecture 31 - Introduction to quantitative proteomics
Lecture 32 - SILAC: In vivo labeling
Lecture 33 - iTRAQ: In vitro labeling
Lecture 34 - TMT: In vitro labeling
Lecture 35 - Quantitative proteomics data analysis
Lecture 36 - Proteomics applications
Lecture 37 - Challenges in proteomics
Lecture 38 - OMICS and translational research
Lecture 39 - Lab session – Targeted proteomics using triple quadrupole mass spectrometry
Lecture 40 - Lab session – Targeted proteomics: multiple reaction monitoring