NOC:Interactomics:Protein Arrays and Label-free Biosensors (USB)

Media Storage Type : 32 GB USB Stick

NPTEL Subject Matter Expert : Prof. Sanjeeva Srivastava

NPTEL Co-ordinating Institute : IIT Bombay

NPTEL Lecture Count : 40

NPTEL Course Size : 28 GB

NPTEL PDF Text Transcription : Available and Included

NPTEL Subtitle Transcription : Available and Included (SRT)

Lecture Titles:

Lecture 1 - Introduction to Interactomics
Lecture 2 - An overview of label-free technologies
Lecture 3 - An overview of surface plasmon resonance (SPR)
Lecture 4 - An overview of surface plasmon resonance imaging (SPRi)
Lecture 5 - Basics of SPR: Surface chemistry
Lecture 6 - Basics of SPR: Experimental design
Lecture 7 - Protein immobilization for protein-protein interaction studies
Lecture 8 - Protein-protein interaction study: Binding analysis
Lecture 9 - Protein-protein interaction study: Kinetic analysis
Lecture 10 - Protein-small molecule interaction study: Immobilization and binding analysis
Lecture 11 - Protein-small molecule interaction study: Kinetic analysis
Lecture 12 - SPR: Interactive Session - I
Lecture 13 - SPR: Interactive Session - II
Lecture 14 - An overview of ellipsometry and interferometry techniques
Lecture 15 - An introduction to BioLayer Interferometry (BLI) and its applications in protein research
Lecture 16 - Kinetic analysis of protein-protein interaction using BLI
Lecture 17 - Label-free quantification of proteins using BLI
Lecture 18 - Diffraction-based biosensors - I
Lecture 19 - Diffraction-based biosensors - II
Lecture 20 - Nanotechniques in proteomics - I
Lecture 21 - Nanotechniques in proteomics - II
Lecture 22 - High throughputplatforms of interactomics: Protein arrays
Lecture 23 - Conventional label based detection techniques for Protein microarrays
Lecture 24 - Novel detection techniques for Protein microarrays
Lecture 25 - Recombinational cloning and its application for Protein microarrays
Lecture 26 - An introduction to Cell-free protein synthesis
Lecture 27 - Cell-free synthesis based protein microarrays: PISA and NAPPA
Lecture 28 - Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays
Lecture 29 - Digging deeper into NAPPA: Basic Workflow
Lecture 30 - Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment
Lecture 31 - Application of cell free expression protein microarrays in biomarker discovery
Lecture 32 - Application of cell free expression protein microarrays in immunological studies
Lecture 33 - Basics of microarray image scanning
Lecture 34 - Software for Image scanning and data processing
Lecture 35 - Microarray Data Analysis - Part I
Lecture 36 - Microarray Data Analysis - Part II
Lecture 37 - Application of protein microarray in biomarker discovery - I
Lecture 38 - Application of protein microarray in biomarker discovery - II
Lecture 39 - Systems biology and networks
Lecture 40 - Challenges in proteomics