NOC:Interactomics: Basics and Applications (USB)

Media Storage Type : 64 GB USB Stick

NPTEL Subject Matter Expert : Prof. Sanjeeva Srivastava

NPTEL Co-ordinating Institute : IIT Bombay

NPTEL Lecture Count : 60

NPTEL Course Size : 58 GB

NPTEL PDF Text Transcription : Available and Included

NPTEL Subtitle Transcription : Available and Included (SRT)

Lecture Titles:

Lecture 1 - Introduction to Proteomics
Lecture 2 - Introduction to Interactomics
Lecture 3 - High throughput platforms of interactomics: Protein arrays
Lecture 4 - Cell-free expression based protein microarrays
Lecture 5 - NAPPA: Recombinational Cloning, Basic workflow, Surface Chemistry, Printing and Assessment
Lecture 6 - NAPPA Technology and Protein Arrays - I
Lecture 7 - NAPPA Technology and Protein Arrays - II
Lecture 8 - Biomarkers: Harnessing the immune system for early detection of disease - I
Lecture 9 - Biomarkers: Harnessing the immune system for early detection of disease - II
Lecture 10 - Biomarkers: Harnessing the immune system for early detection of disease - III
Lecture 11 - NAPPA and its applications in study of antibody immune response in disease and in drug screening - I
Lecture 12 - NAPPA and its applications in study of antibody immune response in disease and in drug screening - II
Lecture 13 - NAPPA and its applications in study of antibody immune response in disease and in drug screening - III
Lecture 14 - Using functional proteomics to identify biomarkers and therapeutic targets - I
Lecture 15 - Using functional proteomics to identify biomarkers and therapeutic targets - II
Lecture 16 - Applications of protein microarrays in Malaria Research - I
Lecture 17 - Applications of protein microarrays in Malaria Research - II
Lecture 18 - Introduction to Bioprinting and IrisOptical QC Benefits - I
Lecture 19 - Introduction to Bioprinting and IrisOptical QC Benefits - II
Lecture 20 - Screening of autoantibody signatures in cancer patients: Lab demonstration
Lecture 21 - Basics of Image Scanning and data acquisition
Lecture 22 - Applications of protein arrays in identification of autoantibody signatures - I
Lecture 23 - Applications of protein arrays in identification of autoantibody signatures - II
Lecture 24 - Applications of protein microarrays in deciphering PTMs and biological networks
Lecture 25 - Basics and Applications of Reverse Phase Protein Arrays - I
Lecture 26 - Basics and Applications of Reverse Phase Protein Arrays - II
Lecture 27 - Basics and Applications of Reverse Phase Protein Arrays - III
Lecture 28 - An overview of label-free technologies
Lecture 29 - Surface Plasmon Resonance - Principles and Assays - I
Lecture 30 - Surface Plasmon Resonance - Principles and Assays - II
Lecture 31 - Basics of SPR: Surface chemistry
Lecture 32 - Basics of SPR: Experimental design
Lecture 33 - Protein immobilization for protein-protein interaction studies
Lecture 34 - Protein-protein interaction study: Binding analysis
Lecture 35 - Protein-protein interaction study: Kinetic analysis
Lecture 36 - Use of SPR in unravelling domain motif interactions of proteasomal assembly chaperones
Lecture 37 - Protein-small molecule interaction study: Immobilization and binding analysis
Lecture 38 - Protein-small molecule interaction study: Kinetic analysis
Lecture 39 - An introduction to biolayer interferometry (BLI)and its applications in protein research
Lecture 40 - Biomolecular interactions using Bio-Layer Interferometry (BLI) - I
Lecture 41 - Biomolecular interactions using Bio-Layer Interferometry (BLI) - II
Lecture 42 - Lab session- An introduction to BioLayer Interferometry (BLI) and its applications in protein research
Lecture 43 - Applications of label-free technologies - II
Lecture 44 - Biomolecular interaction analytics using MicroScale Thermophoresis
Lecture 45 - Mass Spectrometry coupled Interactomics - I
Lecture 46 - Mass Spectrometry coupled Interactomics - II
Lecture 47 - Next-Generation Sequencing Technology - Ion Torrent
Lecture 48 - NGS Technology - Bioinformatics and data analysis - I
Lecture 49 - NGS Technology - Bioinformatics and data analysis - II
Lecture 50 - Next-Generation Sequencing Technology- Illumina
Lecture 51 - Agilent complete NGS target enrichment workflow for exomes, targeted panels and beyond
Lecture 52 - The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome - I
Lecture 53 - The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome - II
Lecture 54 - Statistical Analysis - I
Lecture 55 - Statistical Analysis - II
Lecture 56 - Secondary Data Analysis
Lecture 57 - Pathway Enrichment and Network Analysis
Lecture 58 - Data Repositories and Databases
Lecture 59 - Application of multi-omics approach for better understanding of cancers
Lecture 60 - Integrated Omics and Systems Biology- Conclusion