Media Storage Type : 32 GB USB Stick
NPTEL Subject Matter Expert : Prof. Soumya De, Prof. Swagata Dasgupta
NPTEL Co-ordinating Institute : IIT Kharagpur
NPTEL Lecture Count : 53
NPTEL Course Size : 24 GB
NPTEL PDF Text Transcription : Available and Included
NPTEL Subtitle Transcription : Available and Included (SRT)
Lecture Titles:
Lecture 1 - Introduction
Lecture 2 - Buffers
Lecture 3 - Introduction to Biochemistry Laboratory Equipments and Safety Measures
Lecture 4 - Practical Aspects of Making Buffer
Lecture 5 - Making Tris Buffer (pH=8.2)
Lecture 6 - Making Phosphate Buffer (100mM)
Lecture 7 - Amino Acids and Their Properties
Lecture 8 - Amino Acid Titrations
Lecture 9 - pI Determination of Glycine
Lecture 10 - pI Determination of Lysine
Lecture 11 - Summary
Lecture 12 - UV and Visible Spectroscopy
Lecture 13 - Fluorescence Spectroscopy
Lecture 14 - UV/Visible Spectra of Amino Acids and Proteins
Lecture 15 - Fluorescence Spectra of Amino Acids and proteins
Lecture 16 - Spectroscopic Techniques Summary
Lecture 17 - Protein Folding and Denaturation - I
Lecture 18 - Protein Folding and Denaturation - II
Lecture 19 - Urea denaturation of HSA studied by UV/Vis absorbance
Lecture 20 - Temperature denaturation of HSA studied by UV/Vis absorbance
Lecture 21 - Denaturation of HSA by GdnHCl studied by Trp fluorescence
Lecture 22 - Protein Folding and Denaturation Summary
Lecture 23 - Chromatographic Techniques - I
Lecture 24 - Chromatographic Techniques - II
Lecture 25 - Protein Purification by Size Exclusion Chromatography (SEC)
Lecture 26 - Protein Purification by Affinity Chromatography
Lecture 27 - Gel Electrophoresis of DNA and Proteins - Part I
Lecture 28 - Gel Electrophoresis of DNA and Proteins - Part II
Lecture 29 - Gel Electrophoresis of DNA and Proteins - Part II
Lecture 30 - Isolation and Characterization of Proteins Part - I
Lecture 31 - Isolation and Characterization of Proteins Part - II
Lecture 32 - Isolation and Purification of Proteins
Lecture 33 - Quality and Quantity of the Isolated Protein
Lecture 34 - Enzyme Kinetics - I
Lecture 35 - Enzyme Kinetics - II
Lecture 36 - Enzyme Kinetics (by using enzyme from apple juice)
Lecture 37 - Enzyme Kinetics (by using enzyme from apple juice) (Continued...)
Lecture 38 - Isolation and Characterization of DNA Part - I
Lecture 39 - Isolation and Characterization of DNA Part - II
Lecture 40 - Bacterial Culture for Plasmid DNA Isolation
Lecture 41 - Isolation of Plasmid DNA
Lecture 42 - Isolation and Characterization of DNA Summary
Lecture 43 - Basics of rDNA Technology Part - I
Lecture 44 - Basics of rDNA Technology Part - II
Lecture 45 - Cloning : Polymerase Chain Reaction, Restriction Enzyme Digestion and Ligation
Lecture 46 - DNA Transformation
Lecture 47 - Protein-Ligand Interaction
Lecture 48 - Protein-Ligand Interaction (Continued...)
Lecture 49 - Interaction study of HSA protein with Curcumin and Gallic acid using UV-Vis spectroscopy
Lecture 50 - Interaction study of HSA protein with Circumin and Gallic acid using UV-Vis spectroscopy (Continued...)
Lecture 51 - Analysis of the Structure of Protein ligand complex
Lecture 52 - Immunoassay Techniques
Lecture 53 - Western Blotting Technique