Media Storage Type : DVD-ROM
NPTEL Course Name : Genetic Engineering and Applications
NPTEL Subject Matter Expert : Dr. Utpal Bora
NPTEL Co-ordinating Institute : IIT Guwahati
NPTEL Lecture Count : 59
Lecture Titles:
Lecture 1 - Role of genes within cells, genetic code
Lecture 2 - Method of creating recombinant DNA molecules
Lecture 3 - Types, biology and salient features of vectors in recombinant DNA technology: Plasmids
Lecture 4 - Types, biology and salient features of vectors in recombinant DNA technology
Lecture 5 - Safety guidelines for recombinant DNA research
Lecture 6 - Control of spills and mechanism of implementation of biosafety guidelines
Lecture 7 - Enzymes in genetic engineering: Restriction nucleases: exo and endo nucleases
Lecture 8 - Enzymes in modification- Polynucleotide phosphorylase, DNase and their mechanism of action
Lecture 9 - Enzymes in modification- Methylases and phosphatases and their mechanism of action
Lecture 10 - Enzymes in modification- Polynucleotide kinase, Ligases
Lecture 11 - Methods of nucleic acid detection
Lecture 12 - Polymerase chain reaction (PCR) and its applications
Lecture 13 - Variations in PCR and their applications
Lecture 14 - Methods of nucleic acid hybridization
Lecture 15 - Probe and target sequences
Lecture 16 - Nucleic acid mutagenesis in vivo and in vitro
Lecture 17 - Isolation and purification of nucleic acid (genomic/plasmid DNA and RNA)
Lecture 18 - Quantification and storage of nucleic acids
Lecture 19 - Construction of cDNA library
Lecture 20 - Construction of Genomic library
Lecture 21 - Screening and preservation of DNA libraries
Lecture 22 - Gene transfer techniques: biological methods
Lecture 23 - Gene transfer techniques: chemical methods
Lecture 24 - Gene transfer techniques: physical or mechanical methods
Lecture 25 - Agro- bacterium mediated gene transfer in plants
Lecture 26 - Chloroplast transformation
Lecture 27 - Transgenic science in plant improvement
Lecture 28 - Biopharming - plants as bioreactors
Lecture 29 - Transgenic science for animal improvement
Lecture 30 - Biopharming- Animals as bioreactor for recombinant protein
Lecture 31 - Gene mapping in plants and animals
Lecture 32 - Marker-assisted selection for plant breeding and livestock improvement
Lecture 33 - Microbial biotechnology: Genetic manipulation
Lecture 34 - Engineering microbes for the production of antibiotics and enzymes
Lecture 35 - Engineering microbes for the production of insulin, growth hormones
Lecture 36 - Engineering microbes for clearing oil spills
Lecture 37 - Gene therapy: Introduction and Methods
Lecture 38 - Gene targeting and silencing
Lecture 39 - Gene therapy in the treatment of diseases
Lecture 40 - Challenges and future of gene therapy
Lecture 49 - M1-Problems
Lecture 50 - M2-Problems
Lecture 51 - M3-Problems
Lecture 53 - M5-Problems
Lecture 54 - M6-Problems
Lecture 55 - M7-Problems
Lecture 56 - M8-Problems
Lecture 58 - DNA Sequencing and Cloning Stategies
Lecture 59 - M4-Problems