Media Storage Type : DVD-ROM
NPTEL Course Name : Experimental Biotechnology
NPTEL Subject Matter Expert : Dr. Vishal Trivedi
NPTEL Co-ordinating Institute : IIT Guwahati
NPTEL Lecture Count : 42
Lecture Titles:
Lecture 1 - Introduction
Lecture 2 - Water and aqueous solutions
Lecture 3 - Buffers
Lecture 4 - Determination of protein concentration by ultraviolet spectroscopy
Lecture 5 - Determination of protein concentration by Bradford method
Lecture 6 - Estimation of DNA using diphenylamine method
Lecture 7 - Melting temperature of DNA
Lecture 8 - Equilibrium unfolding of protein
Lecture 9 - Circular dichroism of proteinsï¾–I
Lecture 10 - Circular dichroism of proteinsï¾–II
Lecture 11 - Fourier transform infrared spectroscopy of proteins
Lecture 12 - Enzyme activity
Lecture 13 - Electrophoresis (Part-I)
Lecture 14 - Staining of PAGE Gels
Lecture 15 - Western Blotting
Lecture 16 - Electrophoresis (Part-II)
Lecture 17 - Horizontal Gel Electrohpresis
Lecture 18 - RNA gel
Lecture 19 - Nucleic acid Detection
Lecture 20 - Introduction to chromatography
Lecture 21 - Thin Layer Chromatography
Lecture 22 - Packing a gel filtration column and determination of void volume
Lecture 23 - Protein molecular weight determination using gel filtration chromatography
Lecture 24 - Protein purification using ion exchange chromatography
Lecture 25 - Protein purification using metal chelate affinity (Ni-NTA) chromatography
Lecture 26 - HPLC of proteins and peptides
Lecture 27 - Antibody (Part-I)
Lecture 28 - Antibody (Part-II)
Lecture 29 - Purification of antibody
Lecture 30 - ELISA
Lecture 31 - Light Microscopy
Lecture 32 - Microscopy (Part-II)
Lecture 33 - Microscopy (Part-III)
Lecture 34 - Electron Microscopy - I
Lecture 35 - Electron Microscopy - II
Lecture 36 - Atomic Force Microscopy
Lecture 37 - Preparation of Competent Cells
Lecture 38 - Transformation of Competent Cells
Lecture 39 - Isolation of Recombinant Clones
Lecture 40 - Analysis and Verification of Recombinant Plasmids
Lecture 41 - Mid Semester
Lecture 42 - End Semester