NPTEL : NOC:Interactomics: Basics and Applications (Biotechnology)

Co-ordinators : Prof. Sanjeeva Srivastava


Lecture 1 - Introduction to Proteomics

Lecture 2 - Introduction to Interactomics

Lecture 3 - High throughput platforms of interactomics: Protein arrays

Lecture 4 - Cell-free expression based protein microarrays

Lecture 5 - NAPPA: Recombinational Cloning, Basic workflow, Surface Chemistry, Printing and Assessment

Lecture 6 - NAPPA Technology and Protein Arrays - I

Lecture 7 - NAPPA Technology and Protein Arrays - II

Lecture 8 - Biomarkers: Harnessing the immune system for early detection of disease - I

Lecture 9 - Biomarkers: Harnessing the immune system for early detection of disease - II

Lecture 10 - Biomarkers: Harnessing the immune system for early detection of disease - III

Lecture 11 - NAPPA and its applications in study of antibody immune response in disease and in drug screening - I

Lecture 12 - NAPPA and its applications in study of antibody immune response in disease and in drug screening - II

Lecture 13 - NAPPA and its applications in study of antibody immune response in disease and in drug screening - III

Lecture 14 - Using functional proteomics to identify biomarkers and therapeutic targets - I

Lecture 15 - Using functional proteomics to identify biomarkers and therapeutic targets - II

Lecture 16 - Applications of protein microarrays in Malaria Research - I

Lecture 17 - Applications of protein microarrays in Malaria Research - II

Lecture 18 - Introduction to Bioprinting and IrisOptical QC Benefits - I

Lecture 19 - Introduction to Bioprinting and IrisOptical QC Benefits - II

Lecture 20 - Screening of autoantibody signatures in cancer patients: Lab demonstration

Lecture 21 - Basics of Image Scanning and data acquisition

Lecture 22 - Applications of protein arrays in identification of autoantibody signatures - I

Lecture 23 - Applications of protein arrays in identification of autoantibody signatures - II

Lecture 24 - Applications of protein microarrays in deciphering PTMs and biological networks

Lecture 25 - Basics and Applications of Reverse Phase Protein Arrays - I

Lecture 26 - Basics and Applications of Reverse Phase Protein Arrays - II

Lecture 27 - Basics and Applications of Reverse Phase Protein Arrays - III

Lecture 28 - An overview of label-free technologies

Lecture 29 - Surface Plasmon Resonance - Principles and Assays - I

Lecture 30 - Surface Plasmon Resonance - Principles and Assays - II

Lecture 31 - Basics of SPR: Surface chemistry

Lecture 32 - Basics of SPR: Experimental design

Lecture 33 - Protein immobilization for protein-protein interaction studies

Lecture 34 - Protein-protein interaction study: Binding analysis

Lecture 35 - Protein-protein interaction study: Kinetic analysis

Lecture 36 - Use of SPR in unravelling domain motif interactions of proteasomal assembly chaperones

Lecture 37 - Protein-small molecule interaction study: Immobilization and binding analysis

Lecture 38 - Protein-small molecule interaction study: Kinetic analysis

Lecture 39 - An introduction to biolayer interferometry (BLI)and its applications in protein research

Lecture 40 - Biomolecular interactions using Bio-Layer Interferometry (BLI) - I

Lecture 41 - Biomolecular interactions using Bio-Layer Interferometry (BLI) - II

Lecture 42 - Lab session- An introduction to BioLayer Interferometry (BLI) and its applications in protein research

Lecture 43 - Applications of label-free technologies - II

Lecture 44 - Biomolecular interaction analytics using MicroScale Thermophoresis

Lecture 45 - Mass Spectrometry coupled Interactomics - I

Lecture 46 - Mass Spectrometry coupled Interactomics - II

Lecture 47 - Next-Generation Sequencing Technology - Ion Torrent

Lecture 48 - NGS Technology - Bioinformatics and data analysis - I

Lecture 49 - NGS Technology - Bioinformatics and data analysis - II

Lecture 50 - Next-Generation Sequencing Technology- Illumina

Lecture 51 - Agilent complete NGS target enrichment workflow for exomes, targeted panels and beyond

Lecture 52 - The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome - I

Lecture 53 - The Human Pathology Atlas: A Pathology Atlas of the Human Transcriptome - II

Lecture 54 - Statistical Analysis - I

Lecture 55 - Statistical Analysis - II

Lecture 56 - Secondary Data Analysis

Lecture 57 - Pathway Enrichment and Network Analysis

Lecture 58 - Data Repositories and Databases

Lecture 59 - Application of multi-omics approach for better understanding of cancers

Lecture 60 - Integrated Omics and Systems Biology- Conclusion