NPTEL : NOC:Introduction to Proteomics (Biotechnology)

Co-ordinators : Prof. Sanjeeva Srivastava


Lecture 1 - Introduction to amino acids

Lecture 2 - Introduction to proteins

Lecture 3 - Protein folding and misfolding

Lecture 4 - Introduction to proteomics

Lecture 5 - Lab session – Protein-protein interaction using label-free biosensors

Lecture 6 - Sample preparation and pre-analytical factors

Lecture 7 - Sample preparation: Pre-analytical factors (Continued...)

Lecture 8 - Sample preparation: Protein extraction and quantification

Lecture 9 - One-dimensional electrophoresis

Lecture 10 - Introduction to 2-DE

Lecture 11 - 2-DE: Second dimension, staining and destaining

Lecture 12 - 2-DE: Gel analysis

Lecture 13 - 2-DE Applications

Lecture 14 - 2-DE Applications (Continued...) and Challenges

Lecture 15 - Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR and data analysis

Lecture 16 - 2D-DIGE: Basics

Lecture 17 - 2D-DIGE: Data analysis

Lecture 18 - 2D-DIGE: Applications

Lecture 19 - Systems biology and proteomics - I

Lecture 20 - Systems biology and proteomics - II

Lecture 21 - Fundamentals of mass spectrometry

Lecture 22 - Chromatography technologies

Lecture 23 - Liquid chromatography

Lecture 24 - Mass spectrometry: Ionization sources

Lecture 25 - Mass spectrometry: Mass analyzers

Lecture 26 - MALDI sample preparation and analysis

Lecture 27 - Hybrid mass spectrometry configurations

Lecture 28 - Lab session - Demonstration of Q-TOF MS technology

Lecture 29 - In-gel and in-solution digestion

Lecture 30 - Lab session - Sample preparation: tissue sample preservation technology

Lecture 31 - Introduction to quantitative proteomics

Lecture 32 - SILAC: In vivo labeling

Lecture 33 - iTRAQ: In vitro labeling

Lecture 34 - TMT: In vitro labeling

Lecture 35 - Quantitative proteomics data analysis

Lecture 36 - Proteomics applications

Lecture 37 - Challenges in proteomics

Lecture 38 - OMICS and translational research

Lecture 39 - Lab session – Targeted proteomics using triple quadrupole mass spectrometry

Lecture 40 - Lab session – Targeted proteomics: multiple reaction monitoring