NPTEL : Analytical Technologies in Biotechnology (Biotechnology)

Co-ordinators : Dr. Ashwani K. Sharma


Lecture 1 - Basic concepts in microscopy - 1

Lecture 2 - Basic concepts in microscopy - 2

Lecture 3 - Dark-field and phase contrast microscopy

Lecture 4 - Differential interference contrast and polarization

Lecture 5 - Fluorescence and confocal microscopy

Lecture 6 - Transmission electron microscopy

Lecture 7 - Transmission electron microscopy cont. and scanning electron microscopy

Lecture 8 - Basic concepts - 1

Lecture 9 - Basic concepts - 2

Lecture 10 - GM counting and Scintillation counting

Lecture 11 - Scintillation counting continued

Lecture 12 - Autoradiography and RIA

Lecture 13 - Safety aspects and applications

Lecture 14 - Introduction and Basic concepts in chromatography - 1

Lecture 15 - Basic concepts in chromatography - 2

Lecture 16 - Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography (HPLC)

Lecture 17 - Ion-exchange chromatography

Lecture 18 - Gel-filtration chromatography

Lecture 19 - Affinity chromatography

Lecture 20 - Gas-liquid chromatography

Lecture 21 - Basic concepts in electrophoresis

Lecture 22 - Horizontal and vertical gel electrophoresis

Lecture 23 - Native gel electrophoresis and SDS-PAGE

Lecture 24 - Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods

Lecture 25 - Electrophoresis of nucleic acids

Lecture 26 - Immunoelectrophoresis and capillary electrophoresis

Lecture 27 - Introduction and Basic Concepts - 1

Lecture 28 - Basic concepts - 2

Lecture 29 - Types of centrifuges and analytical ultracentrifugation method

Lecture 30 - Separation methods in preparative ultracentrifuges

Lecture 31 - Types of rotors

Lecture 32 - Types of rotors cont. and care of rotors

Lecture 33 - Introduction and basic concepts

Lecture 34 - UV-Visible spectroscopy

Lecture 35 - Infrared and fluorescence spectroscopy

Lecture 36 - Circular dichroism (CD) spectroscopy

Lecture 37 - Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography

Lecture 38 - Atomic spectroscopy and mass spectrometry

Lecture 39 - Polymerase chain reaction(PCR)

Lecture 40 - DNA sequencing methods

Lecture 41 - Enzyme linked immunosorbent assay (ELISA)