NPTEL : NOC:Genetic Engineering: Theory and Application (Biotechnology)

Co-ordinators : Dr. Vishal Trivedi


Lecture 1 - Cellular Structure - Part I

Lecture 2 - Cellular Structure - Part II

Lecture 3 - Cellular Structure - Part III

Lecture 4 - Metabolic Reactions in Biological System

Lecture 5 - Growth Media For Different Expression System

Lecture 6 - Microbial Growth Kinetics

Lecture 7 - Isolation of a Gene Fragment - Part I

Lecture 8 - Isolation of a Gene Fragment - Part II

Lecture 9 - Isolation of a Gene Fragment - Part III

Lecture 10 - Polymerase Chain Reaction

Lecture 11 - Molecular Tools for Cloning

Lecture 12 - Cloning Vectors - I

Lecture 13 - Cloning Vectors - II

Lecture 14 - DNA Delivery In Host - Part I

Lecture 15 - DNA Delivery In Host - Part II

Lecture 16 - Screening of Recombinant Clones

Lecture 17 - Protein Production in Host - Part 1

Lecture 18 - Protein Production in Host - Part 2

Lecture 19 - Protein Production in Host - Part 3

Lecture 20 - Product Recovery from Host Cells

Lecture 21 - Basics of Chromatography - Part 1

Lecture 22 - Basics of Chromatography - Part 2

Lecture 23 - Ion-exchange Chromatography

Lecture 24 - Hydrophobic Interaction Chromatography

Lecture 25 - Gel Filtration chromatography - Part 1

Lecture 26 - Gel Filtration chromatography - Part 2

Lecture 27 - Affinity Chromatography - Part 1

Lecture 28 - Affinity Chromatography - Part 2

Lecture 29 - Affinity Chromatography - Part 3

Lecture 30 - Affinity Chromatography - Part 4

Lecture 31 - Electrophoresis - Part 1

Lecture 32 - Electrophoresis - Part 2

Lecture 33 - Electrophoresis - Part 3

Lecture 34 - Protein Sequencing

Lecture 35 - Spectroscopy - Part I

Lecture 36 - Spectroscopy - Part II

Lecture 37 - Biotechnology Applications - Part 1

Lecture 38 - Biotechnology Applications - Part 2

Lecture 39 - Biotechnology Applications - Part 3

Lecture 40 - Summary and Conclusions - Part 1

Lecture 41 - Summary and Conclusions - Part 2