NPTEL : NOC:Interactomics: Protein Arrays and Label-free Biosensors (Biotechnology)

Co-ordinators : Prof. Sanjeeva Srivastava


Lecture 1 - Introduction to Interactomics

Lecture 2 - An overview of label-free technologies

Lecture 3 - An overview of surface plasmon resonance (SPR)

Lecture 4 - An overview of surface plasmon resonance imaging (SPRi)

Lecture 5 - Basics of SPR: Surface chemistry

Lecture 6 - Basics of SPR: Experimental design

Lecture 7 - Protein immobilization for protein-protein interaction studies

Lecture 8 - Protein-protein interaction study: Binding analysis

Lecture 9 - Protein-protein interaction study: Kinetic analysis

Lecture 10 - Protein-small molecule interaction study: Immobilization and binding analysis

Lecture 11 - Protein-small molecule interaction study: Kinetic analysis

Lecture 12 - SPR: Interactive Session - I

Lecture 13 - SPR: Interactive Session - II

Lecture 14 - An overview of ellipsometry and interferometry techniques

Lecture 15 - An introduction to BioLayer Interferometry (BLI) and its applications in protein research

Lecture 16 - Kinetic analysis of protein-protein interaction using BLI

Lecture 17 - Label-free quantification of proteins using BLI

Lecture 18 - Diffraction-based biosensors - I

Lecture 19 - Diffraction-based biosensors - II

Lecture 20 - Nanotechniques in proteomics - I

Lecture 21 - Nanotechniques in proteomics - II

Lecture 22 - High throughputplatforms of interactomics: Protein arrays

Lecture 23 - Conventional label based detection techniques for Protein microarrays

Lecture 24 - Novel detection techniques for Protein microarrays

Lecture 25 - Recombinational cloning and its application for Protein microarrays

Lecture 26 - An introduction to Cell-free protein synthesis

Lecture 27 - Cell-free synthesis based protein microarrays: PISA and NAPPA

Lecture 28 - Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays

Lecture 29 - Digging deeper into NAPPA: Basic Workflow

Lecture 30 - Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment

Lecture 31 - Application of cell free expression protein microarrays in biomarker discovery

Lecture 32 - Application of cell free expression protein microarrays in immunological studies

Lecture 33 - Basics of microarray image scanning

Lecture 34 - Software for Image scanning and data processing

Lecture 35 - Microarray Data Analysis - Part I

Lecture 36 - Microarray Data Analysis - Part II

Lecture 37 - Application of protein microarray in biomarker discovery - I

Lecture 38 - Application of protein microarray in biomarker discovery - II

Lecture 39 - Systems biology and networks

Lecture 40 - Challenges in proteomics