NPTEL : NOC:Proteins and Gel-Based Proteomics (Biotechnology)

Co-ordinators : Prof. Sanjeeva Srivastava


Lecture 0 - Proteins and Gel-Based Proteomics; Course Introduction

Lecture 1 - Introduction to amino acids

Lecture 2 - Introduction to proteins

Lecture 3 - Protein folding & misfolding

Lecture 4 - Protein purification techniques

Lecture 5 - Introduction to proteomics

Lecture 6 - Systems biology and proteomics

Lecture 7 - Sample preparation and pre-analytical factors

Lecture 8 - Sample preparation: Pre-analytical factors (Continued...)

Lecture 9 - Sample preparation: Protein extraction and quantification

Lecture 10 - One-dimensional electrophoresis

Lecture 11 - 2-DE: Rehydration, IEF & Equilibration

Lecture 12 - 2-DE: Second dimension, staining & destaining

Lecture 13 - 2-DE: Gel analysis

Lecture 14 - 2-DE: Applications

Lecture 15 - 2-DE: Applications (Continued...) & Challenges

Lecture 16 - 2D-DIGE: Basics

Lecture 17 - 2D-DIGE: Data analysis

Lecture 18 - 2D-DIGE: Applications

Lecture 19 - Protein identification using MALDI-TOF/TOF

Lecture 20 - Proteomics experiment data analysis & challenges